UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSAN, ETIL ASETAT DAN AIR DARI EKSTRAK ETANOLIK DAUN UNGU (Graptophyllum pictum (L.) Griff) TERHADAP Staphylococcus aureus ATCC 25923

Suryaku, Nora Ilham (2017) UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSAN, ETIL ASETAT DAN AIR DARI EKSTRAK ETANOLIK DAUN UNGU (Graptophyllum pictum (L.) Griff) TERHADAP Staphylococcus aureus ATCC 25923. Skripsi thesis, Universitas Setia Budi Surakarta.

[img] Text
INTISARI NORA pdf.pdf

Download (226kB)
[img] Text
SKRIPSI NORA pdf.pdf

Download (6MB)

Abstract

Infection disease is one of the biggest health problems in developing countries, one of which is in Indonesia. Staphylococcus aureus is the cause of 70% of cases of nosocomial infections and causes of pyogenic infections. Purple leaf are known to contain flavonoids, saponins, tannins, alkaloids, steroids. The purpose of the study was to determine the activity of n-hexane, ethyl acetate, and water fractions from ethanol extract purple leaf (Graptophyllum pictum (L.) Griff) as antibacterial against S. aureus ATCC 25923. The purple leaf powder was extracted using a maceration method with 96% ethanol solvent, then fractionated using n-hexane, ethyl acetate and water solvents with different polarities. The test of the antibacterial activity was performed by using diffusion concentration 50%, 25%, and 12,5%. Then, the most active fractions were diluted to determine MIC and MKC using concentrations of 50%, 25%, 12.5%, 6.25%, 3.125%, 1.563%, 0.781%, 0.391%, 0.196%, 0.098%. Statistic alanalysis in this study using ANOVA one way to determine whether there is a significant difference from the test preparation. The results of antibacterial activity test of extract, n-hexane, ethyl acetate, and water fractions with diffusion method at concentration 50% resulted in inhibition zone of 12.99 mm; 11.33 mm; 17.11 mm; And 12.77 mm. The results showed that all fractions and extracts had antibacterial activity. The ethyl acetate fraction of purple leaf’s ethanol extract has the most active antibacterial activity against S. aureus ATCC 25923 with MKC value of 12.5%. The results of phytochemical identification showed ethyl acetate fraction containing flavonoids, alkaloids, and steroids. Keywords: Antibacterial, staphylococcus aureus, purple leaf, fractionation, diffusion, dilution, Graptophyllum pictum (L). Griff

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: Antibacterial, staphylococcus aureus, purple leaf, fractionation, diffusion, dilution, Graptophyllum pictum (L). Griff
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas Farmasi > Prodi S1 Farmasi
Depositing User: Herera Gadys USB
Date Deposited: 25 Feb 2019 03:00
Last Modified: 25 Feb 2019 03:00
URI: http://repository.setiabudi.ac.id/id/eprint/982

Actions (login required)

View Item View Item