UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSANA, ETIL ASETAT DAN AIR DARI EKSTRAK ETANOL DAUN BINAHONG (Anredera cordifolia (Ten.) Steenis) TERHADAP Escherichia coli ATCC 25922

Andhini, Nisa Fitri (2017) UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSANA, ETIL ASETAT DAN AIR DARI EKSTRAK ETANOL DAUN BINAHONG (Anredera cordifolia (Ten.) Steenis) TERHADAP Escherichia coli ATCC 25922. Skripsi thesis, Universitas Setia Budi Surakarta.

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Abstract

Binahong leaf (Anredera cordifolia (Ten.) Steenis) are species of crops which good to heal some kinds of diseases. Binahong leaves contains flavonoid, alkaloid, saponin and terpenoid that have antibacterial activity. This study aims to determine the antibacterial activity of the fraction of n-hexane, ethyl acetate and water from binahong leaves ethanol extract against Escherichia coli ATCC 25922. Binahong leaves powder was extracted by maceration method by ethanol 70%, and then it was fractionated by solvent n-hexane, ethyl acetate and water which have different polarity. Antibacterial activity test was performed using diffusion and dilution methods. The concentration used in the diffusion method was 50%, 25% and 12,5% aimed to determine the most active fraction. The most active fraction is continued dilution test to determine the MBC (Minimum Bactericidal Concentration) with concentration 50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78%, 0.39%, 0.19%, 0.09%. Statistical analysis using oneway ANOVA to determine whether there is a significant difference between the test preparation. The results shows that all the fractions and extracts of Anredera cordifolia (Ten.) Steen had antibacterial activity. Water fraction is most active fraction with concentration of 50% (19.1 mm), concentration of 25% (15.83 mm) and concentration of 12.5% (13.26 mm). Dilution test results water fraction showed antibacterial activity with MBC 6.25%. The results of phytochemical identification showed fractions of water containing flavonoid, alkaloid and saponin. Keywords : Binahong leaves, fraction of n-hexane, ethyl acetate fraction, fraction of water, Escherichia coli.

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: Binahong leaves, fraction of n-hexane, ethyl acetate fraction, fraction of water, Escherichia coli.
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas Farmasi > Prodi S1 Farmasi
Depositing User: Herera Gadys USB
Date Deposited: 25 Feb 2019 02:59
Last Modified: 25 Feb 2019 02:59
URI: http://repository.setiabudi.ac.id/id/eprint/976

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