IDENTIFIKASI DAN SKRINING AKTIVITAS ANTIBAKTERI DAN ANTIJAMUR EKSTRAK ETANOL RIMPANG KUNCI PEPET (Kaempferia rotunda L.)

Fauziah, Dina Trianggaluh (2016) IDENTIFIKASI DAN SKRINING AKTIVITAS ANTIBAKTERI DAN ANTIJAMUR EKSTRAK ETANOL RIMPANG KUNCI PEPET (Kaempferia rotunda L.). Tesis thesis, Universitas Setia Budi Surakarta.

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Abstract

Kaempferia rotunda L. is a medicinal plant from ginger family which is locally known as kunci pepet. K. rotunda L. contains many secondary metabolites including essential oils, flavonoids and terpenoids, which can act as therapy for dysentery, diarrhea, and also for body slim treatment. The extraction method used in this research was maceration. Then the current extract was further fractionated using n-hexane and dichloromethane. Those fractions were tested for its antibacterial and antifungal activities using agar diffusion method. Identification of the qualitative content of secondary metabolites is carried out by thin layer chromatography (TLC). Inhibition zones of ethanol extract, hexane, dichloromethane and aqueous fraction against Escherichia coli and Staphylococcus aureus were 2.08 cm, 1.37 cm, 1.47 cm, 1.27 cm, and 2.13 cm, 1.30 cm, 1.30 cm, 1.57 cm, respectively. The inhibition zones of the crude extract, fractions of n- hexane, dichloromethane and aqueous fraction are 1.82 cm, 1, 27 cm, 1.50 cm, 1.33 cm. GCMS analysis of the extract, fraction of n-hexane, dichloromethane and aqueous fractions showed that K. Rotunda L.,contains benzoic acid (8.97%), hexadecane (28.84% and 33.50%), benzyl benzoate (89.21%, 14.71% , 7.23% and 19.88%), 2-hydroxy benzoic acid ester phenilmethil (0.90%), heksadekanoat acid (0.92%, 10.10%, 7.04% and 11.71%), benzyl alcohol (12.30 %), pentadekana (7.22%), octadecanoic methyl ester (5.43%). Based on LCMS analysis K. Rotunda L. also contains crotepoxide, zeylenol epoxide, zeylenol, and 6-methyl zeylenol Keyword : Kunci Pepet, Fractionation, GCMS, LCMS.

Item Type: Thesis (Tesis)
Uncontrolled Keywords: Keyword : Kunci Pepet, Fractionation, GCMS, LCMS.
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas Farmasi > Prodi S2 Farmasi
Depositing User: Herera Gadys USB
Date Deposited: 23 Feb 2019 03:19
Last Modified: 23 Feb 2019 03:19
URI: http://repository.setiabudi.ac.id/id/eprint/663

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