UJI AKTIVITAS SITOTOKSIK EKSTRAK DAN FRAKSI - FRAKSI DAUN JAMBU BIJI (Psidium guajava L.) TERHADAP SEL KANKER PAYUDARA MCF-7

Widyasari, Eva (2019) UJI AKTIVITAS SITOTOKSIK EKSTRAK DAN FRAKSI - FRAKSI DAUN JAMBU BIJI (Psidium guajava L.) TERHADAP SEL KANKER PAYUDARA MCF-7. Skripsi thesis, Universitas Setia Budi Surakarta.

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Abstract

Guava leaf (Psidium guajava L.) is a plant parts Myrtaceae efficacious to inhibit the growth of cancer, antiviral, antibacterial, and anti-diarrhea. This study aims to determine the cytotoxic activity of extracts and fractions of guava leaves, selectivity index, class of chemical compounds and fractions most active. Plant leaves of guava extracted using maceration method with ethanol and fractionated with n-hexane, ethyl acetate and water. Cytotoxic activity tests were carried out using the MTT (Microculture Tetrazolium Technique) method with a concentration series of 1000; 500; 250; 125; 62.5; 31.25; 15,625 μg/mL. Cisplatin positive control using concentration series 100; 50; 25; 12.5; 6.25; 3,125; 1.5625 μg/mL. IC50 values were obtained from the linear regression equation between log concentration vs% cell viability. Cytotoxic activity against breast cancer cells MCF-7 obtained IC50 values of ethanol extract, n-hexane fraction, ethyl acetate fraction and water fraction respectively 320.4401 μg/ml, 71.473 μg/ml, 202.163 μg/ml and 244,054 μg/ml. Selectivity index ethanol extract of 4.04, n-hexane fraction of 8.52, ethyl acetate fraction of 6.6, and a water fraction of 12.11. Groups of chemical compounds suspected of having cytotoxic activity are steroids, alkaloids, and tannins. N-hexane is the most active fraction. Keywords: Guava leaves (Psidium guajava L.), MCF-7 cells, cytotoxics fraction of n-hexane, ethyl acetate and water

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: Guava leaves (Psidium guajava L.), MCF-7 cells, cytotoxics fraction of n-hexane, ethyl acetate and water
Subjects: Q Science > QK Botany
R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas Farmasi > Prodi S1 Farmasi
Depositing User: Mrs NOVI HANDAYANI
Date Deposited: 13 Feb 2020 03:35
Last Modified: 13 Feb 2020 03:35
URI: http://repository.setiabudi.ac.id/id/eprint/4092

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