UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 70%, FRAKSI n-HEKSANA, ETIL ASETAT DAN AIR DAUN KARI (Murraya koenigii (L.) Spreng) TERHADAP BAKTERI Staphylococcus aureus ATCC 25923 SECARA in vitro

Prasilia, Risha Ayu (2019) UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 70%, FRAKSI n-HEKSANA, ETIL ASETAT DAN AIR DAUN KARI (Murraya koenigii (L.) Spreng) TERHADAP BAKTERI Staphylococcus aureus ATCC 25923 SECARA in vitro. Skripsi thesis, Universitas Setia Budi.

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Abstract

Curry leaves contain some chemical compounds serving as antibacterial agent: flavonoids, tannins, saponins, alkaloids and steroids. This research aimed to find out the antibacterial activity of 70% ethanol extract, n-hexane, ethyl acetate and water fractions of curry leaves, to find out which one has most effective antibacterial activity, whether ethanol extract or three fractions of curry leaves, and to find out Minimum Inhibiting Concentration (MIC) and Minimum Killing Concentration (MKC) produced by the most active 70% ethanol extract of curry leaves on Staphylococcus aureus ATCC 25923. The antibacterial activity test in this study was conducted using diffusion and dilution methods. Diffusion method to obtain the inhibition zone diameter from extract and fraction. The diameter of the inhibition zone was analyzed using ANOVA data. The dilution method was carried out in a series of dilution of the most active fraction of ethyl acetate to obtain the MIC value, after which inoculation was carried out to obtain the KBM value. 70% ethanol extract, n-hexane, ethyl acetate and water fraction of curry leaves have antibacterial activity. The most active fraction of ethyl acetate is 40% with inhibiting zone diameter 17.16 mm. The Minimum Killing Concentration value of ethyl acetate fraction is 5%. . Key words : Curry leaves, Fractionation, Staphylococcus aureus ATCC 25923.

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: Curry leaves, Fractionation, Staphylococcus aureus ATCC 25923
Subjects: Q Science > QR Microbiology
Divisions: Fakultas Farmasi > Prodi S1 Farmasi
Depositing User: Tifany Nur Arfiana
Date Deposited: 14 Nov 2019 04:42
Last Modified: 14 Nov 2019 04:42
URI: http://repository.setiabudi.ac.id/id/eprint/3855

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