PENAPISAN BAKTERI PENGHASIL ENZIM SUPEROKSIDA DISMUTASE (SOD) DARI AIR HUTAN MANGROVE MARON EDUPARK SEMARANG

Saptarini, Diyah (2019) PENAPISAN BAKTERI PENGHASIL ENZIM SUPEROKSIDA DISMUTASE (SOD) DARI AIR HUTAN MANGROVE MARON EDUPARK SEMARANG. Skripsi thesis, Universitas Setia Budi.

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Abstract

Superoxide dismutase (SOD) is one of the body's produced antioxidant enzymes. The role of inhibiting free radical reactivity that damages the structure and function of cells. SOD enzymes can be obtained from microbes derived from mangrove forest water. The mangrove ecosystem is a source of various microbes capable of producing useful enzymes and molecules. This research aims to find out which bacteria produce SOD enzymes and know the highest type of SOD producing species. The selection of 5 isolates of bacteria with different characteristics macroscopic performed before the test of SOD activity. Measuring activity using an assay kit WST-1. The principle of knowing SOD's ability catalyzes the dismutase of the superoxide anions into hydrogen peroxide and oxygen molecules. SOD's activity is known from the percentage value SOD. The isolates of bacteria that have the highest SOD activity performed Gram and PCR 16S rDNA coloration. The results of SOD activity test are isolates AHM5 (45.10%), AHM2 (49.02%), AHM1 (56.86%), AHM3 (66.67%), and AHM4 (80.39%). Bacterial isolates AHM4 carried out identification of Gram staining and PCR 16S rDNA. The result of identification by coloration of Gram isolates AHM4 is Grampositive bacteria. The result of determining the order of nucleotide isolates of bacteria AHM4 obtained the identity of Bacillus Cereus with a homology of 99%. Based on the search for the Uniprot site Bacillus Cereus is known to be registered as a protein producer SOD. Keywords: SOD activity, Bacillus Cereus, Mangrove Forest, superoxide Dismutase

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: SOD activity, Bacillus Cereus, Mangrove Forest, superoxide Dismutase
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas Farmasi > Prodi S1 Farmasi
Depositing User: Tifany Nur Arfiana
Date Deposited: 01 Nov 2019 03:37
Last Modified: 01 Nov 2019 03:37
URI: http://repository.setiabudi.ac.id/id/eprint/3628

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