EKSTRAK DAN FRAKSI TEH HIJAU (Camellia sinensis L. Kuntze.) SEBAGAI ANTIBIOFILM TERHADAP BAKTERI Pseudomonas aeruginosa ATCC 27853 DAN Staphylococcus aureus ATCC 25923

Wardani, Tatiana Siska (2019) EKSTRAK DAN FRAKSI TEH HIJAU (Camellia sinensis L. Kuntze.) SEBAGAI ANTIBIOFILM TERHADAP BAKTERI Pseudomonas aeruginosa ATCC 27853 DAN Staphylococcus aureus ATCC 25923. Tesis thesis, Universitas Setia Budi Surakarta.

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Abstract

This study, potency of extract and fraction (water, n-hexan, etyl acetat) from green tea leaves to inhibit biofilm formation and to degradade of biofilm produce by P. aeruginosa ATCC 27853 and S. aureus ATCC 25923 were evaluated. Total phenolic content of green tea leaves was determined using spectrofotometry UV visible on λ=646 nm. Green tea leaves were macerated with 96% ethanol then were fractionated with water, ethyl acetate and n-hexane solvents. DO value (optical density) optimization, inhibition and degradation of biofilms were carried out through the microtiter plate method (using 96 wells), followed by using a microplate reader on λ = 595 nm. Then, total phenolic content of the extract and fraction by spectrophotometry were determined. The results showed that IC50 value from extracts, water fraction, ethyl acetate fraction and n-hexane to inhibit P. aeruginosa ATCC 27853 biofilms were 1.353; 1.389; 1.203, and 1.481 mg/mL respectively and the IC50 value for S. aureus ATCC 25923 is 2,319; 4,046; 2,548 and 2,297 mg / ml. EC50 values of extract, water fraction, ethyl acetate fraction and n-hexane to degrade P. aeruginosa ATCC 27853 biofilms were 3.069; 1.930; 1.910; and 2.158 mg/mL and while the EC50 value of S. aureus ATCC 25923 is 3.329; 3,327; 4,140 and 3,284 mg / mL. Total phenolic contains from extracts, water, ethyl acetate, and n-hexane fractions were 8.97; 6.729; 7.26; and 7.82 %. These results indicate that green tea leave has the potential antibiofilm for P. aeruginosa ATCC 27853. Key word : Green tea, antibiofilm, P. aeruginosa ATCC 27853, S. aureus ATCC 25923

Item Type: Thesis (Tesis)
Uncontrolled Keywords: Green tea, antibiofilm, P. aeruginosa ATCC 27853, S. aureus ATCC 25923
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas Farmasi > Prodi S2 Farmasi
Depositing User: Mrs NOVI HANDAYANI
Date Deposited: 01 Nov 2019 03:40
Last Modified: 01 Nov 2019 03:40
URI: http://repository.setiabudi.ac.id/id/eprint/3472

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