AKTIVITAS FRAKSI DAN SUBFRAKSI EKSTRAK ETANOL DAUN MANGGA BACANG (Mangifera foetida L.) TERHADAP KADAR FERITIN DARAH PASIEN TALASEMIA β MAYOR SECARA IN VITRO DAN KUANTIFIKASI KANDUNGAN MANGIFERIN DALAM SUB FRAKSI AKTIF

Erikania, Susanti (2019) AKTIVITAS FRAKSI DAN SUBFRAKSI EKSTRAK ETANOL DAUN MANGGA BACANG (Mangifera foetida L.) TERHADAP KADAR FERITIN DARAH PASIEN TALASEMIA β MAYOR SECARA IN VITRO DAN KUANTIFIKASI KANDUNGAN MANGIFERIN DALAM SUB FRAKSI AKTIF. Tesis thesis, Universitas Setia Budi Surakarta.

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Abstract

Thalassemia β major was one of thalassemia with severe anemia so patients need blood transfusion continuous ly which causes organ dysfunction due to iron accumulation. Patients must had been using iron chelator therapy to reduce iron levels in the body. Iron chelatorwas expensive and had many side effects. The M. foetida leaves was thought to contain mangiferin which had chelating effect. This study aims to determine the activity of fractions and sub fractions from M. foetida leaves ethanol extract to decreasing ferritin levels in blood serum of major β thalassemia patients and quantification mangiferin in active sub fraction. Fractions and sub fractions of M. foetida leaves ethanol extract of were obtained by ECC and KCV which determined their activity using Elisa ferritin method by in vitro. Detection of mangiferin in ECC fractions using TLC with stationary phase of silica gel 60 F254 and 3 mobile phases was chloroform: ethanol: glacial acetic acid (8: 2: 1), ethyl acetate: formic acid: water (8: 0, 5: 0.5) and n butanol: acetic acid : water (10 : 1,5: 1) compared to the mangiferin standard. Determination of the mangiferin content in active sub fraction of KCV used densitometry with the stationary phase of silica gel F254 and the mobile phase of ethyl acetate: formic acid : water (8 : 0.5 : 0,5) at a wavelength of 254 nm. The results of this study was indicated that the fractions of ECC named ethyl acetate fraction at concentration of 500 ppmwas able to reduce ferritin levels in blood serum as the highest were74,41 ± 0,17 %% compared to ethanol extract was 47,89 ± 0,20 % and dichloromethane fraction was 8,62 ± 0,27 %. Detection of mangiferin in ethyl acetate fraction shows the same Rf value as mangiferin standard was 0,22. The sub fractions ofKCV namedsub fraction B at concentration of 50 ppm was able to reduce ferritin levels the highest by 88,16 ± 0,61% contained mangiferin at 4,04 ± 0,01 μg / ml. Keywords: Major β thalassemia, Ferritin, Elisa, Mangiferin and Densitometry

Item Type: Thesis (Tesis)
Uncontrolled Keywords: Major β thalassemia, Ferritin, Elisa, Mangiferin and Densitometry.
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas Farmasi > Prodi S2 Farmasi
Depositing User: Mrs NOVI HANDAYANI
Date Deposited: 01 Nov 2019 07:29
Last Modified: 01 Nov 2019 07:29
URI: http://repository.setiabudi.ac.id/id/eprint/3458

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