PENGARUH PEMBERIAN FRAKSI n-HEKSAN BUAH TAKOKAK (Solanum torvum Swartz) TERHADAP PROLIFERASI LIMFOSIT TIKUS MODEL MULTIPLE SCLEROSIS

SARA, Rahmawati (2015) PENGARUH PEMBERIAN FRAKSI n-HEKSAN BUAH TAKOKAK (Solanum torvum Swartz) TERHADAP PROLIFERASI LIMFOSIT TIKUS MODEL MULTIPLE SCLEROSIS. Skripsi thesis, Universitas Setia Budi Surakarta.

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Abstract

Multiple sclerosis (MS) is a chronic neurodegenerative disease of the central nervous system (CNS) because of the attack of innate and adaptive immune system that causes demyelination, loss of oligodendrocyte, axonal degeneration and inflammation. MS is caused by autoreactive lymphocytes against CNS. Imunosupresant effects contained in takokak fruit (Solanum torvum, Swartz.), expected to be used to suppress the proliferation of lymphocytes in MS. This research aimed to determine the effect of n-hexane fraction of takokak fruit and the most effective dose on lymphocyte proliferation of MS rat. Rats were divided into 7 groups, each group consisting of 5 rats. Group 1 as the normal control group, group 2 as the negative control were given Na CMC 0.5% and group 3 as the positive control were given methyl prednisolone 45 mg/kg BW. Groups 4, 5, 6 and 7 are given n-hexane fraction with successive doses; 70 mg/kgBW, 140 mg/kgBW, 280 mg/kgBW and 560 mg/kgBW. On day 0, groups of 2-7 induced with 0.2 ml encephalitogen sc and 0.5 ml Pentabio® vaccines sc. On 3th day, groups of 2-7 induced with 0.5 ml Pentabio® vaccine sc. Groups 2-7 were given the test preparation from day to 14-30. Each day, the rats were weighed and observed the neurologic symptoms. Lymphocyte proliferation test using MTT assay. The proliferation results are read using an ELISA reader at wavelength of 550 nm. Data were analyzed by one way ANOVA using SPSS. The results of lymphocyte proliferation test showed that the fraction of nhexane takokak fruit (Solanum torvum, Swartz.), has an effect in supress the lymphocytes proliferation of rat model of MS with the effective dose is 140 mg/kg BW. Keywords: n-Hexane fraction, Takokak Fruit (Solanum torvum, Swartz.), Multiple Sclerosis (MS), MTT Assay, Lymphocyte Proliferation

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: n-Hexane fraction, Takokak Fruit (Solanum torvum, Swartz.), Multiple Sclerosis (MS), MTT Assay, Lymphocyte Proliferation
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas Farmasi > Prodi S1 Farmasi
Depositing User: Mrs NOVI HANDAYANI
Date Deposited: 21 Feb 2020 03:21
Last Modified: 21 Feb 2020 03:21
URI: http://repository.setiabudi.ac.id/id/eprint/2363

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