UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL, FRAKSI n-HEKSANA, ETIL ASETAT DAN AIR DAUN BUGENVIL (Bougainvillea spectabilis) TERHADAP Pseudomonas aeruginosa ATCC 27853

ASUK, Brigita Maria Febrina (2018) UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL, FRAKSI n-HEKSANA, ETIL ASETAT DAN AIR DAUN BUGENVIL (Bougainvillea spectabilis) TERHADAP Pseudomonas aeruginosa ATCC 27853. Skripsi thesis, Universitas Setia Budi.

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Abstract

Bougainvillea (Bougainvillea spectabilis) leaf has chemical contents of flavonoid, alkaloid, tannin, saponin and triterpenoids suspected of having antibacterial activity. This study is aims to determine the ability of ethanol extract, fraction of n-hexane, ethyl acetate and water from the Bougainvillea leaf (family Nyctaginaceae) against Pseudomonas aeruginosa ATCC 27853. Bougainvillea leaf was extracted using percolation method with 70% ethanol and then fractionated using n-hexane, ethyl acetate and water. Extract and fractions was tested its antibacterial activity using diffusion and dilution method. Diffusion method with a concentration of 50%, 25%, 12.5% and a positive control cotrimoxazole 4,8 mg. Dilution method using serial dilutions of 50%, 25%, 12.5 %, 6.25 %, 3.12%. 1.56%, 0.78%, 0.39%, 0.1 %, 0.09%. The result of this study showed that the fraction of ethyl acetate has the best antibacterial activity than the n-hexane fraction, water fraction and leaf ethanol extract of bougainvillea leaf. The best activity in ethyl acetate fraction of the ethanol extract of bougainvillea leaf at concentration of 50%, with inhibition 17.33 mm. MCK(Minimum Killing Concentration) fraction of ethyl acetate extract of bougainvillea leaf ethanol to kill Pseudomonas aeruginosa ATCC 27853 is a concentration of 3.12%. Keyword: Bougainvillea leaf, fractionation, antibacterial assay, Pseudomonas aeruginosa ATCC 27853

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: Bougainvillea leaf, fractionation, antibacterial assay, Pseudomonas aeruginosa ATCC 27853
Subjects: R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions: Fakultas Farmasi > Prodi S1 Farmasi
Depositing User: Tifany Nur Arfiana
Date Deposited: 22 Feb 2019 08:08
Last Modified: 22 Feb 2019 08:08
URI: http://repository.setiabudi.ac.id/id/eprint/197

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