UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSAN, ETIL ASETAT, DAN AIR DARI EKSTRAK ETANOLIK DAUN BINAHONG (Anredera cordifolia (Tenore) Steen.) TERHADAP BAKTERI Staphylococcus aureus ATCC 25923 DAN Pseudomonas aeruginosa ATCC 27853 SECARA In Vitro

Ngobe, Serfia (2017) UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSAN, ETIL ASETAT, DAN AIR DARI EKSTRAK ETANOLIK DAUN BINAHONG (Anredera cordifolia (Tenore) Steen.) TERHADAP BAKTERI Staphylococcus aureus ATCC 25923 DAN Pseudomonas aeruginosa ATCC 27853 SECARA In Vitro. Skripsi thesis, Universitas Setia Budi Surakarta.

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Abstract

Binahong (Anredera cordifolia (Tenore) Steen.) leaf have chemical contents of flavonoid and saponin are thought to have antibacterial activity. The purposeof this studywas to determine theactivity of ethanolicextract, n-hexane, ethyl acetate andwater fractions from Binahong (Anredera cordifolia (Tenore) Steen) asantibacterial of Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853. Binahong leaf extracted by maserationusing 70% ethanol then fractionated using n-hexane, ethyl acetate and water solvents. Ethanolic extract, n-hexane, ethyl acetate and water fractions were tested the antibacterial activity using diffusion method, with concentration of 50%, 25%, 12,5%. The results showed that the n-heksan fraction, ethyl acetate fraction, and water fraction binahong leaf has antibacterial activity against Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853 at a certain concentration. Inhibitory average diameter extract, n-hexane fraction, ethyl acetate fraction and water fraction with the concentration 50% at Staphylococcus aureus respectively, 12 mm, 12.33 mm, 15.33 mm, dan 14 mm.. Inhibitory average diameter with the concentration 50% at Pseudomonas aeruginosa respectively 12.33 mm, 13.33 mm, 16.67 mm, dan 14.33 mm. The most active fraction against Staphylococcus aureus and Pseudomonas aeruginosa is the ethyl acetate fraction. Keywords: binahong leaf, Staphylococcus aureusATCC25923 and Pseudomonas aeruginosa ATCC 27853 ,diffusion method.

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: binahong leaf, Staphylococcus aureusATCC25923 and Pseudomonas aeruginosa ATCC 27853 ,diffusion method.
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas Farmasi > Prodi S1 Farmasi
Depositing User: Herera Gadys USB
Date Deposited: 25 Feb 2019 03:39
Last Modified: 25 Feb 2019 03:39
URI: http://repository.setiabudi.ac.id/id/eprint/1221

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