UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSANA, KLOROFORM, DAN AIR DARI EKSTRAK METANOL BUAH BELIMBING WULUH (Averrhoa bilimbi L.) TERHADAP Shigella dysenteriae ATCC 9361

RAMADHANI, Shintha (2018) UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSANA, KLOROFORM, DAN AIR DARI EKSTRAK METANOL BUAH BELIMBING WULUH (Averrhoa bilimbi L.) TERHADAP Shigella dysenteriae ATCC 9361. Skripsi thesis, Universitas Setia Budi Surakarta.

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Abstract

The fruit of bilimbi has contains flavonoid, saponin, triterpenoid, and tannin compounds which have antibacterial activity against Gram positive and Gram negative bacteria. The purpose of this study was to test the antibacterial activity of n-hexane, chloroform, and water fractions from methanol extract of bilimbi fruit (Averrhoa bilimbi L.) against Shigella dysenteriae ATCC 9361. The extraction of bilimbi fruit using percolation method with methanol as solvent, and then the fractionation was use the different level polarity diluents. The results of extraction and fractionations was tested of antibacterial activity agains Shigella dysenteriae ATCC 9361 using diffusion method. The consentration of methanol extract and fractions was 400 mg/ml; 200 mg/ml; 100 mg/ml; 50 mg/ml; 25 mg/ml. The result of this study showed that methanol extract, n-hexane, chloroform, and water fractions was have antibacterial activity against Shigella dysenteriae ATCC 9361. The result of the diffusion test shows that the fraction that have the most affective activity against Shigella dysenteriae ATCC 9361 was fraction of choloroform, with average inhibitory diameter 20,3 mm. Key words : bilimbi fruit, fractionation, Shigella dysenteriae ATCC 9361, antibacterial.

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: bilimbi fruit, fractionation, Shigella dysenteriae ATCC 9361, antibacterial.
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas Farmasi > Prodi S1 Farmasi
Depositing User: Tifany Nur Arfiana
Date Deposited: 25 Feb 2019 03:31
Last Modified: 25 Feb 2019 03:31
URI: http://repository.setiabudi.ac.id/id/eprint/1175

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